An efficient method to identify virus-specific TCRs for TCR-T cell immunotherapy against virus-associated malignancies

An efficient method to identify virus-specific TCRs for TCR-T cell immunotherapy against virus-associated malignancies

Blog Article

Abstract Adoptive transfer of T cells genetically engineered with a T cell receptor (TCR) is a promising cancer treatment modality that requires the identification of TCRs with good characteristics.Most T cell cloning methods involve a stringent singularization process, which Hayward C250 necessitates either tedious hands-on operations or high cost.We present an efficient and nonstringent cloning approach based on existing techniques.We hypothesize that after elimination of most nonspecific T cells, a clonotype with high quality could outcompete other clonotypes and finally Advanced Wound Care - Composites form a predominant population.This TCR identification method can be used to clone virus-specific TCRs efficiently from cancer patients and is easily adoptable by any laboratory.